"How can macromolecular interactions be engineered in a rational manner to have the desired effects in a complex environment, such as a living organism?"
Answering this question is a major focus of much of the work carried out in the Green Lab. The interactions between biological macromolecules, and particularly proteins, play a fundamental role in biology, and while we have learnt a great deal about these interactions on many levels, our understanding is not yet at the level needed for the robust engineering of novel macromolecules. We are working on multiple fronts: to push forward the level of general understanding of biomolecular interections; to develop novel computational tools for the study and design of interacting macromolecules; and to directly apply our expertise to the rational design and engineering of proteins with real-world applicablity.
A major focus is on answering questions related to specificity:
Our choice of methods to solve these problems are primarily the tools of computational biology. We employ a range of models and computational methods, including:
Additionally, we currently are beginning an experimental component to our work, expressing, purifying and characterizing proteins which we have designed through rational computer-aided engineering.
The Green Lab is an actived member of a number of interdisciplinary groups that bring together scientists from a range of Stony Brook departments, as well as individuals at Brookhaven National Laboratory. For students interested in graduate study, we are affiliated with several graduate programs which suit students from diverse backgrounds. We are additionally affiliated with summer research programs for undergraduates and high-school students who are interested in gaining hands-on experience in research.